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No holos are virgins because Cover has taken up the practice of whoring out their talents to high paying investors and business partners. This is actually the reason why Ayame does nto stream often, since she is in very high demand and therefore has more value in prostituting herself than in streaming.
I can confirm this because I have taken part in this practice before and have fucked all of EN. Although I must admit that when it came to Kiara it's more apt to say that she fucked me, that girl is wild.