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Not related to the Bible, but there's a less dogmatic, metaphysical justification for refusing to jerk off: fapping concentrates your thoughts on an object of lust and activates your innermost animalistic instincts, which turns you away from your human rational nature. Even if you're fapping to a fictional character, you're still hankering after a physical part of the body you'd like to fuck in your fantasies. It doesn't matter whether it's real or fake, what matters is that you're listening to your primal instincts instead of displaying self-control and appealing to the rational nature that you were endowed with at birth.