https://www.nature.com/articles/d41586-022-01620-3>The long-term evolution experiment (LTEE) has become a cornerstone in evolutionary biology that researchers continue to mine for insights. During their 75,000 generations of growth, the bacteria have made huge gains in their fitness — how fast they grow relative to other bacteria — and evolved some surprising traits.https://journals.asm.org/doi/10.1128/JB.00831-15>The LTEE isolation of Cit+ mutants has become a textbook example of the power of long-term evolution to generate new species. But, based on our results, E. coli arrives at the same solution to access citrate in days versus years, as originally shown by Hall. In either case, genes involved in the process maintain their same function but show expanded expression by deregulation. Because of this, we argue that this is not speciation any more than is the case with any other regulatory mutant of E. coli.>No new genetic information (novel gene function) evolved.No...new...genetic...information.