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OP, there are a few bike related anime: Yowamushi Pedal and Overdrive are your typical sports anime series. Nasu: Summer in Andalusia is a great little film made by former Ghibli staff that also has a sequel film (Wataridori). And there's Hill Climb Girl, a short animation. Besides those, there are a few episodes that involve cycling (more than just featuring characters riding mamacharis, which happens in lots of anime) in Golden Boy, Honey and Clover, and The Tatami Galaxy. Finally, the Longriders anime adaption is supposed to start airing October 8.
