I got all my Morchella importuna cultures done yesterday, I have plates of tissue from sclerotia, from spore, and from the stem. I'll be damned if I don't get atleast one that takes. I did 12 total. Running it on PDA. I have heard it can colonize a plate in just two days, so by tomorrow I am hoping to be amazed.
I also went ahead and inoculated agar with spores from Agrocybe praecox, a woodloving species that is often ignored as being edible, but is one of my personal favorites.
>>995640>>995658You will usually get around 3 flushes if you handle humidity well. You can in fact turn that one block into many more afterwards. Since the block comes with grain in it, you can stretch it out with carbon quite a lot, but will eventually exhaust the grain and need to figure out how to supplement nitrogen in (usually with grain) without getting contamination, and that is where people begin investing in pressure cookers and sterile setups.
The carbon part of its diet can be met easily with coffee grounds, straw, or hardwood sawdust. That allows you to split your kit easily. Don't really need any sterile setup for those portions. (break the block up and mix it in) Eventually though the grain it came with will have run out, and it either wont fruit, or it will stop colonizing.
Grain unfortunately molds if even a few airborne spores get inside, and is already contaminated, which makes that part a lot harder. People usually mix wheat bran into sawdust as their substrate, and the mixture must be pasteurized somehow. Usually with a pressure cooker, but some people get away with steam methods or microwaving.
And like this
>>995811 anon said, you can take wild mushrooms and develop cultures through spores, stem butts, or tissue culture on agar. Stem butts are the only viable option for a beginner who doesn't want to make any investment, and works best with wood-loving species like Oyster mushrooms and Shiitakes.
