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So, today I inoculated two bags with black oyster mushroom grain spawn which I made using a liquid culture. Since I've heard oyster mushrooms can digest pretty much any wood-based substrates, I have one bag filled with sawdust and coco coir, and the other one filled with just regular cardboard from some old boxes to test comparative yields. However, I did supplement with a little bit of used coffee grounds in each one. I weighed approximately 1 kg of dry substrate, fully hydrated it, pasteurized it in the oven for a few hours, and then filled up the bags with cooled substrate and grain spawn. Since my pressure cooker is too small to sterilize this much substrate, and I'm using a pretty poor, makeshift laminar flow hood to reduce contamination, I added the pasteurized coffee grounds together with the grains spawn so the oysters can quickly spread to it in case any contaminants fell into the substrate.
I'm also attempting to grow enoki mushrooms, king oysters, and golden oysters. The king oyster spawn is doing well and was transferred to more grain today, but I'm not seeing much going on with the enoki and golden oyster so far.
