>>2363694I'm not going for fancy scientific set up, I don't even have a scope lol. Was gonna do all of this work in my sab. The only materials I don't currently have are the 15mil tubes and disposable pipettes to make the dilutions. The videos that Gary made for the breeding project I never remembered seeing him bring out a scope to confirm, but maybe he mentioned doing this in one of the videos and I just missed it because of the reasons I mentioned earlier on why I haven't finished the series completely. So if its possible to do it without a scope I'd like to try it out and see if I can get away with it in my sab.
>to produce f1s at a marginally better rateIts not just for that purpose, if I have a bunch of mono isolates from a list of varieties I can now easily just take transfers and mix and match onto a plate. So not only does it increase my odds of viable mating to happen but I can now try out even more different crosses with better chances. At least that's how I understood the benefits of serial dilution, correct me if I'm wrong.
>your cross projectDid you go the typical route of stabilizing or did you try experimenting with something new? The typical route from what I read is that after you have your cross on agar, you then spawn it and pick a fruit you think has some traits of both varieties(usually not apparent at f1), take some spore from that, grow it out again and repeat the process until about f7 when its starting to uniformly show traits of both strains and then you have your cross. Right? So I'm wondering is if there's any other nuances to stabilizing a cross, or is what I outlined really all there is to it?
>you don't need to geek outI'm planning to try all methods; serial dilution, swabbing agar, mixing spawn. Basically just wanted to know if a scope is totally necessary for SD so that I don't have to wait a few months until I get my own scope to start up the project
