>>3949769Thanks. Increasing contrast is a tough issue when it comes to microscopes. There are a few solutions, I'm working on one right now.
In general one can do interference contrast, contrast staining, fluorescence microscopy or immuncytochemical marking. Or just make transgenic shit with GFP, but the plasmids are expensive and you can only use it ons something already in culture.
For now i'm just going to do DIC, where i take two polar filters, put one under the stage between the condensor and the light source and one above the stage where the head atatches to the arm. Rotate them at a 90 degree angle and only polarised light comes through. That increases contrast and makes membranes more pronounced.
Unfortunatly getting polar filters the right size and shape and quality is not cheap or easy.
Also, here, have a blade of grass.
