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Items are, again, similar to those in Pokémon Mystery Dungeon. They're akin to Expedition cards but are used by a specific Pokémon, and use up that Pokémon's action for the turn. They can have a variety of effects. A few Items are not single-use but multi-use, but in general they'll be used and resolved. Certain Items can be used as a reaction, and some Items have additional effects if they are thrown rather than used.
If a Pokémon has not moved during its turn, it can move to an adjacent lane in response to an attack by an opponent Pokémon facing an empty lane. If Opponent Pokémon is in Lane A, and you have no Pokémon in Lane A but one in Lane B who has not yet gone that turn, Pokémon B can move into Lane A to take the attack.
Expedition cards represent events that occur during an Expedition and take on a similar role to Trainer cards. Some may be cast instantaneously, others only as a reaction. One thing to note is that the "Stack"/"Chain" exists here, wherein players can respond to others' actions by activating their own abilities or cards, which is resolved on a last-in-first-out/first-in-last-out basis. Expedition cards typically are not placed in lanes since they are resolved and then are discarded, but Expedition cards can also be placed face-down in lanes and activated later (see below), oftentimes to play mindgames with the opponent or to safeguard from hand-discard effects. Some Expedition cards may occasionally be made permanent and those would go into an appropriate lane.