>>81609299Mumei belongs in C, Fauna in A. Have you read Fauna's papers? she's highly intelligent. see below:
>Actinomycetes are valuable bacteria because they produce many biological productsthat we can use. As a result, the isolation of actinomycetes from the environment
has been a focus of research for decades. In this experiment, we exposed dilutions of
soil sample taken from a small glade to different treatments and then plated them on
different media with different antibiotics to determine which media and treatment
combination gave the highest relative percentage of actinomycetes. We selected 15
strains from the environmental plates and characterized them via an analysis of their
morphology, mycelia, spore production, 16s rDNA sequences, and antimicrobial
production. Generally, the HV medium was the best for actinomycete enumeration
with the HV-Nal/NHS combination giving the highest relative percentage of
actinomycetes. Our analysis of the selected environmental strains revealed that all 15
strains are distinct (but not definitively identified) Streptomyces species. All 15 strains
had characteristic actinomycete morphology. One of our key observations to arise from
this study is that while the environmental isolation media chosen did not often result
in a high relative percentage of actinomycetes, they did enable the isolation of a diverse
subgroup of Streptomyces species. However, the lack of isolation of non-streptomycete
actinomycetes could indicate that these media are not as effective at isolating a diverse
group of actinomycetes. Our results suggest that these methods are effective for diverse
actinomycete isolation, which will be beneficial for isolating novel species in the future.
