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This could be the end of SubaLuna pair. Luna openly said on Choco's anniversary that Subaru cheered her up when she's feeling down, even brought her to a date in Minecraft when she couldn't attend the Usaken Summer Fez due to KroObi live. And now Subaru's not doing so well yet Luna hasn't tweeted or talked about it. Judging from how Subaru tweeted that Ayame cheered her up with a hilarious joke, there's a big chance Luna hasn't reached out to her in private yet. I know the whole yuriteetee is just an act, but not returning the flavor when one's in need could seriously mess up the trust between each other.
They'll most likely still remain friends, but it won't feel as genuine when Luna tries to tease Subaru with suggestive moves.