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What these EN members need to realize is what Holo was built on....CGDCT.
That's the strength the power that pushed Holo to where it is today. If you're picking up a Yuri anime you don't expect to see a man become part of the relationship or when you pick up a BL anime you don't expect to see a women enter the triangle......That's the same for Holo.
The audience has become accustomed to same sex members collaborating because it's what they want to see.
Of course with this you get Unicorns and parasocial fags along the wave but I don't think it makes up the majority of the Holo audience but it comes part and parcel with V-Tubing because how close fan interaction is.
It's like going to a curry house and getting served Pizza, That's not what you came for. People don't come to Holo to watch collabs with males and it'll always be that way because that's how it was built. (I know some will throw the old 2019 Nijisanji collabs with Ayame etc but that was when Holo had no identity and a bunch of nobodies.)
You can collab with males if you want, It's not forbidden but just remember they didn't start watching Holo to see that kind of stuff, That's why HolostaJP has completely failed because the audience isn't there.