Nene doing 350 situps in one stream, nearly dying from hyperventilating, scaring most of her fans and apologizing profusely on her Twitter, with it being her most disliked stream (afaik)
I love my retarded wife, but I was there when it happened and it's so far been the only time a vtuber has given me genuine fear and anxiety.
https://youtu.be/g63fOCTQ2zY 