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I dont think every holo needs to be a playable character.
Kaela for example could be the weapon shop npc. Choco handles the clinic which works like a DQ church. Subaru handles the INN and there's a cooking sidequest in the inn's kitchen involving Aki and Chammers. AZKi is the inn's songstress and has some sidequest to introduce mechanics to players.
Fubuki, Mio, Ayame and Iroha are dlc sidequest in THE EASTERN LANDS about repairing the barrier to stop Anya from becoming japanese. All 5 unlocked after completion.
Okayu and Korone are recurring incompetent baddies, that the party befriends after they figure out they just want food. Luna is part of the party in the beginning but gets kidnapped (yes like in lufia), to figure out her connection to the Moon.
Aqua works in countess Mel's castle as a gamer maid (recruitable if you solve her sidequest of cleaning up the castle). Noel also works there as a guard, barring entry to the castle until you recruit flare and have her as the leading character and talk to Noel.
Antagonist of the game is Pekora who is trying to find a way to go to the moon by using a volatile spell to explode a part of holoearth off and catapult herself over to the moon. Earthquakes in the testing phase alert the initial party to investigate.
Pekora is reluctantly aided by Marine who becomes a recruitable partymember if you defeat her with aqua in the party.
La+ tries to impede your progress and help Pekora, but Pekora doesn't even know she exists.
Miko (healer) is part of the Party from the start, along with Shion (mage), Polka (thief) and Sora as the hero.
Watame is recruitable early on as a bard type. Kanata as a monk type.
Nene is too storonk so she is only obtainable later on as a summon, usable once per fight, empties mp bar fully. Nene dai bakushou plays during the summon, ends with "restroom time".
Towa and Botan sidequest involving helping either in a shooting competition. Unocks one first, the other can be unlocked later. Towa provides heals and buffs while having a ranged attack, Botan is a pure gunner type.
Lamy is a questgiver, which introduces the pure springs to the player, which serve as save points, heal spots and warps later on after you find lamy's secret ingredient. She can be found in the inn at the bar.
Lui can be recruited after the first time you defeat La+. She hangs around town and keeps mentioning finding yamada.
Koyori will sell potions. Can you handle her strongest potions?
Risu is a questgiver and asks you to find nuts (collectible, found throughout the whole world)
Iofi needs help finding colors for her painting project, rewards you with an out of combat ability to open sealed paths, by painting on them with a magic brush.
Ollie is the boss of the graveyard area.
Reine can be summoned after you find a feather of hers to fly you over certain obstacles or as a combat summon.
Kobo is every annoying child in the town
Chloe is the water summon, that can be used in combat or the overworld to cross streams of water or the sea (to get to the EASTERN LANDS for example)
I know nothing about Zeta so no idea what to do with her.
Moona is the final boss, the moon presence who orchestrated everything and turned pekora into the villain. Pekora is recruitable after you defeat and spare her, otherwise the moon presence takes over your mind and it's game over.
Rushia is a secret boss and unlockable character in a difficult sidedungeon.
Coco is a super hard, secret bossfight after you finish the game.
Matsuri is a joke character that just stands in front of an occupied bathroom saying, that she has been holding it in for days.
No en, fuck em.