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During the Lazulight/Obsydia era in 2021, if you added up all the NijiEN girl's streams during primetime, you would get about 8k-8.5k. This is also what they got for the Meltdown collab, and Selen's birthday.
About 2k of these viewers were unique to Selen, so they could only hit a collective total of 6k if Selen wasn't streaming.
Furthermore, as they reported to us, their male fans were 90% of the audience.
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This means that in 2021, NijiEN had a male audience pool of about 7.5k or so. The debut of Ethyria did not change this number even a bit, they just split some viewers with the other girls.
During the current era in 2023, all of the NijiEN girl's streams added together during NA primetime (peak male audience) will yield about 5.5k.
This total can increase if Enna is streaming, although she rarely streams in the NA timeslot, but it's important to note that over half of Enna's viewers are female.
For the Lazulight/Obsydia girls, about 70% of their current-day fans are male, and this is much lower for the ILuna girls.
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This means that in 2023, NijiEN has a male audience pool of about 3.5k - 4k. There has been a huge decline in the male fanbase, for obvious reasons. This is quite alarming, because male fans constitute the majority of the donors, members, people who actually pay money to the girls. Case in point, Enna's handcam streams get massive viewers, sometimes as high as 8k, but her donations do not move one iota. Her small contingent of 1-2k male fans are financially supporting her.
NijiEN has lost about half their male fans since 2021, despite copes to the contrary. The only person who has managed to buck this trend is Scarle, who actually has a significant number of male fans that are unique to her and don't watch anyone else. That's because Scarle has a model that appeals to males (boobs), and uses lewd thumbnails that appeal to males. More importantly, Scarle avoids collabs and interactions with her coworkers. She's able to exist in isolation away from the horrible clique and the males.