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Any girl who remains in HoloEN is redelegating herself to a secondary character in a branch she helped build, just like what happened to Pomu and her crew after Luxiem debuted in NijiEN. After maletubers are introduced into a branch the girls become mere appendages and accessories to the males. Decorations. Their career, content and fans (members, SC) are inextricably linked to the male they associate with the most. They lose all independent agency and control over their fanbase and content, and thus they lose their personalities as well. I have seen it happen with Niji, and now it's happening in HoloEN.
Is it better to be a single person creating content within a company, or being a single person whose content is beholden to ANOTHER person within the company AND the company itself?
There are degrees of independence and interdependence, and girls who collab with men gave up control of a huge part of their independence and increased their interdependence towards two parties, the maletuber and the company. This means much less bargaining power, much more dependence on the maletuber, much less independent decision making and as such much less personality in terms of content and feel of their streams. This all translates to a much weaker relationship with her fans (membership, SC), content that depends on the maletuber AND the company (many things become impossible to do, your ideas carry less weight, your impact is lesser), and of course no matter what you do or do not do will always be seen through your interdependence with the maletuber. This usually translates to "people" - here in quotes because they're fujoshi - hating you and wanting you dead and/or as quiet and grey as a mouse. Which loops back into the girl having less of a presence and personality than before, which them means less and less fans because you no longer are you, but a bland and tasteless nobody.
tl;dr every female chuuba in HoloEN who helped out maletubers and collabed with them has done irreparable damage to herself.