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I'll copypaste something I wrote on another forum a while back, it was for anime with badass mature women :
- 009-1
- Afro Samurai: Resurrection - (Sio, main antagonist)
- Basilisk (Akeginu, Kagerou)
- Berserk (Casca)
- Bubblegum Crisis (Sylia)
- Cleopatra D.C.
- Code Geass (Cornelia, Villetta
- Cowboy Bebop (Faye Valentine
- Darker than Black (Misaki Kirihara)
- Death Parade
- Ghost Sweeper Mikami
- Gundress
- Iria: Zeiram the Animation
- Jojo's Bizarre Adventure (2012) (Lisa Lisa, although she only appears in the 2nd half)
- Kill la Kill (Ragyou Kiryuuin, main antagonist for the 2nd half)
- Lupin III (Fujiko)
- Madlax
- Maps
- Michiko & Hatchin (Michiko)
- Najica Blitz Tactics
- Noir
- Penguindrum (Yuri Tokikago, antagonist)
- Phantom: Requiem for the Phantom (Claudia McCunnen)
- Phantom Quest Corps
- Princess Mononoke (can't deny Lady Eboshi is badass)
- R.O.D. (OVA) (Nancy Makuhari)
- Record of Lodoss War (OVA, Karla is the main antagonist for a good bunch of the anime IIRC)
- Resident Evil: Damnation (Ada Wong, Mrs. President)
- Ryoko's Case File
- Silent Mobius
- Street Fighter II: The Animated Movie (Chun-Li)
- Super Street Fighter IV (Juri, Chun-Li, Cammy)
- Tekken: Blood Vengeance (Nina, Anna, they get sidelined by younger girls later though)
- Valkyria Chronicles (Selvaria, kinda the Dragon but still)
- Vampire Hunter
