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so I have a problem about determining the CFU/ml of my sample. My professor gave a formula to determine cfu/ml which is colony count/ (total dilution of tube X amount plated). We used two plates for every serial dilution. I dont understand if I should multiply the total dilution of tube (1/10^-x) to the amount plated (1 dilution is plated on 2 plates) since the "amount plated" was defined as "the amount of dilution used to make the
particular pour plate or spread plate" which would be 1. What do?
particular pour plate or spread plate" which would be 1. What do?