>>92322Ruroni Kenshin
Kenshin is like, 34 or 35. though he looks like he's still in his 20s (played for laughs in a couple of offhand lines) season 1 and 2 are pretty good, season 3 starts getting ridiculous pretty fast though...
it's a manga, but BERSERK features a heavily grizzled main character in what I'd assume is his mid to late 30s.
Ghost in the Shell. the major is in a young body, but she is far from young
granted, I'm not counting anime where characters are a supernaturally old, but justify their nature to act like children such as Hellsing or [spoiler:lit]Trigun[/spoiler:lit]
thats the best I got, what do you have on your list already?
I'm curious...