Okay, so, as someone who watched the actual stream and a bigtime fan of Mousey, yes, he fucked up pretty bad. It was just a tone-deaf and insensitive thing to ask, but he also didn't know what it was, and he has been providing Mousey with some tech support on shit so he may have had a legit concern that her mic was bad or something was causing interference. It was an unfortunate coincidence for both of them. Mousey is sensitive about her condition and her stuff, but she is also really good friends with the guy and knows he didn't mean to do that. Connor just kept digging a hole spilling his spaghetti all over the place because he does care about her, too. Despite the tsundere antagonistic act they put up with each other, they're good friends.
Arguably the more damning thing he did was later when he dropped an indication that he's not only well aware of Mousey's condition, but may have seen some of the physical parts of her life she has to deal with. IE, he's seen her IRL and her machines she uses and other stuff. Which embarrassed her even more even tho I don't think he picked up on what his gaffe meant. Chat picked up on it immediately and were curious but go no further clarification.
It was on stream, so it was public, and it was embarrassing for them both, but there's no reason to go further than that. She doesn't hate him for it and he's not going to run away from her out of shame. They're still good friends and there's a lot of love between them. It's water under the bridge.
Of course, if you /vt/ards want to use it as a weapon against Connor, go ahead, I can't stop you, but anybody else without a beef, this was an awkward one time thing and not worth harping on.